Incubation:Article Title: G Protein Coupled Estrogen Receptor Signaling Maintains β Cell Identity in Female Mice
Article Snippet: .. Following deparaffinization, rehydration, and antigen retrieval by heating in a citrate antigen retrieval buffer (H-3300-250, Vector Laboratories), sections were permeabilized, blocked with Animal-Free Blocker (SP-5030-250, Vector Laboratories), and incubated with primary antibodies overnight at 4 °C (Insulin – Dako, A0564, RRID:AB_10013624, Glucagon – Abcam, ab92517, RRID:AB_10561971, Ki67 – Cell Signaling Technology, #9129, RRID:AB_2687446). .. Incubation with secondary antibodies conjugated to AlexaFluor (Thermo Fisher) was performed at room temperature for 1.5 hours.
Article Title: ΔNp63 Regulates Homeostasis, Stemness, and Suppression of Inflammation in the Adult Epidermis.
Article Snippet: .. For immunohistochemistry, slides were boiled in citrate antigen retrieval buffer (Vector Laboratories, Newark, CA) for 15 minutes, blocked with 5% normal goat serum (Life Technologies, Carlsbad, CA), and incubated overnight at 4 C with primary antibodies: p63 (1:750, catalog number 13109, Cell Signaling Technology, Danvers, MA) and Ki-67 (1:400, catalog number 12202, Cell Signaling Technology). .. Slides were washed in PBS and incubated with biotinylated secondary antibody (Invitrogen), followed by Vectastain Elite ABC peroxidase reagent (Vector Labs) and DAB Quanto substrate (Thermo Fisher Scientific, Waltham, MA) with hematoxylin counterstain.
Immunohistochemistry:Article Title: ΔNp63 Regulates Homeostasis, Stemness, and Suppression of Inflammation in the Adult Epidermis.
Article Snippet: .. For immunohistochemistry, slides were boiled in citrate antigen retrieval buffer (Vector Laboratories, Newark, CA) for 15 minutes, blocked with 5% normal goat serum (Life Technologies, Carlsbad, CA), and incubated overnight at 4 C with primary antibodies: p63 (1:750, catalog number 13109, Cell Signaling Technology, Danvers, MA) and Ki-67 (1:400, catalog number 12202, Cell Signaling Technology). .. Slides were washed in PBS and incubated with biotinylated secondary antibody (Invitrogen), followed by Vectastain Elite ABC peroxidase reagent (Vector Labs) and DAB Quanto substrate (Thermo Fisher Scientific, Waltham, MA) with hematoxylin counterstain.
Article Title: Inhibition of FGFR signaling with infigratinib improves linear bone growth in the female
<i>Aga2/+</i>
mouse model of osteogenesis imperfecta
Article Snippet: .. For IHC, sections were rehydrated, heated in citrate antigen retrieval buffer (Vector Laboratories, H3300) at 95 °C for 20 minutes, quenched in methanol with 3% H2O2 for 10 minutes, then stained using either anti-phospho-ERK1/2 (Cell Signaling Technology; 9101; 1:250) or anti-SOX9 (Cell Signaling Technology; 82630; 1:500) overnight at 4 °C. .. Slides were then incubated with biotinylated anti-rabbit IgG (Vector Laboratories, BP-9100-50) followed by streptavidin peroxidase (Vector Laboratories, SA-5704) for 30 minutes each before incubation with 3,3′-diaminobenzidine tetrahydrochloride (Vector Laboratories, SK-4105).
Article Title: Missing link between tissue specific expressing pattern of ERβ and the clinical manifestations in LGBLEL
Article Snippet: .. For IHC staining, sections were deparaffinized and rehydrated with a histoclear/alcohol series and antigen retrieval was performed by boiling in 1 × citrate antigen retrieval buffer (Vector), endogenous peroxidases were blocked by treating slides with 3% hydrogen peroxide for 15 min at room temperature. .. Slides were blocked in PBS with 5% normal goat serum (Bioss, C0005) and primary antibody staining was performed overnight in PBS with 1% goat serum at 4 °C.
Article Title: Inhibition of FGFR signaling with infigratinib improves linear bone growth in the female Aga2/+ mouse model of osteogenesis imperfecta
Article Snippet: .. For immunohistochemistry (IHC), sections were rehydrated and heated in citrate antigen retrieval buffer (Vector Laboratories, H3300) at 95 °C for 20 min, quenched in methanol with 3% H 2 O 2 for 10 min, and then stained using either anti-phospho-ERK1/2 (Cell Signaling Technology; 9101; 1:250) or anti-SOX9 (Cell Signaling Technology; 82630; 1:500) overnight at 4 °C. .. Slides were then incubated with biotinylated anti-rabbit IgG (Vector Laboratories, BP-9100-50) followed by streptavidin peroxidase (Vector Laboratories, SA-5704) for 30 min each before incubation with 3,3′-diaminobenzidine tetrahydrochloride (Vector Laboratories, SK-4105).
Staining:Article Title: Inhibition of FGFR signaling with infigratinib improves linear bone growth in the female
<i>Aga2/+</i>
mouse model of osteogenesis imperfecta
Article Snippet: .. For IHC, sections were rehydrated, heated in citrate antigen retrieval buffer (Vector Laboratories, H3300) at 95 °C for 20 minutes, quenched in methanol with 3% H2O2 for 10 minutes, then stained using either anti-phospho-ERK1/2 (Cell Signaling Technology; 9101; 1:250) or anti-SOX9 (Cell Signaling Technology; 82630; 1:500) overnight at 4 °C. .. Slides were then incubated with biotinylated anti-rabbit IgG (Vector Laboratories, BP-9100-50) followed by streptavidin peroxidase (Vector Laboratories, SA-5704) for 30 minutes each before incubation with 3,3′-diaminobenzidine tetrahydrochloride (Vector Laboratories, SK-4105).
Article Title: Missing link between tissue specific expressing pattern of ERβ and the clinical manifestations in LGBLEL
Article Snippet: .. For IHC staining, sections were deparaffinized and rehydrated with a histoclear/alcohol series and antigen retrieval was performed by boiling in 1 × citrate antigen retrieval buffer (Vector), endogenous peroxidases were blocked by treating slides with 3% hydrogen peroxide for 15 min at room temperature. .. Slides were blocked in PBS with 5% normal goat serum (Bioss, C0005) and primary antibody staining was performed overnight in PBS with 1% goat serum at 4 °C.
Article Title: Inhibition of FGFR signaling with infigratinib improves linear bone growth in the female Aga2/+ mouse model of osteogenesis imperfecta
Article Snippet: .. For immunohistochemistry (IHC), sections were rehydrated and heated in citrate antigen retrieval buffer (Vector Laboratories, H3300) at 95 °C for 20 min, quenched in methanol with 3% H 2 O 2 for 10 min, and then stained using either anti-phospho-ERK1/2 (Cell Signaling Technology; 9101; 1:250) or anti-SOX9 (Cell Signaling Technology; 82630; 1:500) overnight at 4 °C. .. Slides were then incubated with biotinylated anti-rabbit IgG (Vector Laboratories, BP-9100-50) followed by streptavidin peroxidase (Vector Laboratories, SA-5704) for 30 min each before incubation with 3,3′-diaminobenzidine tetrahydrochloride (Vector Laboratories, SK-4105).
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